|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
Application of quantitative analysis of multi-component system approach for determination of ginsenosides in different mass-spectrometric conditionsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 ноября 2018 г.
- Авторы: Elena Stekolshchikova, Polina Turova, Oleg Shpigun, Igor Rodin, Andrey Stavrianidi
- Журнал: Journal of Chromatography A
- Том: 1574
- Год издания: 2018
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 82
- Последняя страница: 90
- DOI: 10.1016/j.chroma.2018.09.005
- Аннотация: The lack of standard substances is a bottleneck for quality control in traditional and alternative medicine. Development of the HPLC-UV approaches combined with single standard for quantitative analysis of multi-component system (QAMS) allowed determination of several plant biomarkers by implementation of the relative response factors (RRFs). Robustness and ruggedness of such methods are commonly demonstrated by performing the analysis in changing analytical conditions on the different HPLC equipment and columns. The nature of MS detection is much more complicated and dependent on the instrumentation. Therefore, this study was conducted to justify the use of RRFs for HPLC-MS determination of bioactive compounds from plants. Protopanaxatriol (PPT), protopanaxadiol (PPD) and ocotillol (OT) ginseng saponins (ginsenosides) were successfully separated on a reversed-phase PFP-column with high group selectivity. Fragmentation patterns for these groups of compounds were established on different HPLC-ESI-MS systems and at varied declustering potentials (DPs). The use of sapogenin fragmentation ions in positive detection mode and group reference standards was shown to be an optimal way to perform quantification. The performance of the developed group targeted HPLC-MS-QAMS approach was tested in the course of measurements conducted on the different instrumentation. The differences between QAMS and external standard method (ESM) quantification results were below 15% for all determined saponins.
- Добавил в систему: Ставрианиди Андрей Николаевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|