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Tma64/eIF2D, Tma20/MCT-1, and Tma22/DENR Recycle Post-termination 40S Subunits In Vivoстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 декабря 2018 г.
- Авторы: Young David J., Makeeva Desislava S., Zhang Fan, Anisimova Aleksandra S., Stolboushkina Elena A., Ghobakhlou Fardin, Shatsky Ivan N., Dmitriev Sergey E., Hinnebusch Alan G., Guydosh Nicholas R.
- Журнал: Molecular Cell
- Том: 71
- Номер: 5
- Год издания: 2018
- Издательство: Cell Press
- Местоположение издательства: United States
- Первая страница: 761
- Последняя страница: 774.e5
- DOI: 10.1016/j.molcel.2018.07.028
- Аннотация: The recycling of ribosomal subunits after translation termination is critical for efficient gene expression. Tma64 (eIF2D), Tma20 (MCT-1), and Tma22 (DENR) function as 40S recycling factors in vitro, but it is unknown whether they perform this function in vivo. Ribosome profiling of tma deletion strains revealed 80S ribosomes queued behind the stop codon, consistent with a block in 40S recycling. We found that unrecycled ribosomes could reinitiate translation at AUG codons in the 3' UTR, as evidenced by peaks in the footprint data and 3' UTR reporter analysis. In vitro translation experiments using reporter mRNAs containing upstream open reading frames (uORFs) further established that reinitiation increased in the absence of these proteins. In some cases, 40S ribosomes appeared to rejoin with 60S subunits and undergo an 80S reinitiation process in 3' UTRs. These results support a crucial role for Tma64, Tma20, and Tma22 in recycling 40S ribosomal subunits at stop codons and translation reinitiation.
- Добавил в систему: Дмитриев Сергей Евгеньевич