Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells

Keremidarska-Markova, M.; Radeva, E.; Mitev, D.; Hristova-Panusheva, K.; Paull, B.; Nesterenko, P.; Sepitka, J.; Junkar, I.; Iglic, A.; Krasteva, N.

Авторы: Keremidarska-Markova Milena, Radeva Ekaterina, Mitev Dimitar, Hristova-Panusheva Kamelia, Paull Brett, Nesterenko Pavel, Sepitka Joseph, Junkar Ita, Iglic Ales, Krasteva Natalia
Журнал: Turkish Journal of Biology
Том: 42
Номер: 2
Год издания: 2018
Издательство: Scientific and Technical research Council of Turkey - TUBITAK/Turkiye Bilimsel ve Teknik Arastirma Kurumu
Местоположение издательства: Turkey
Первая страница: 195
Последняя страница: 203
DOI: 10.3906/biy-1711-26
Аннотация: In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2-2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hAD-MSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er') with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.
Добавил в систему: Нестеренко Павел Николаевич

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Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cellsстатья

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