Dependence of immobilized alpha-chymotrypsin activity in water-organic solvent mixtures upon composition of microsurroundings (rigid matrix and hydrating additives)статья
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Аннотация:Dependence of catalytic activity of alpha-chymotrypsin immobilized on viscose in isopropanol-water mixtures (water content < 10%) on the water content was investigated. An addition of a cyclic polyether, 18-crown-6, to the solution of alpha-chymotrypsin before immobilization was found to lead to a significant (7 to 10 times) increase of enzymic activity in the system studied, and this activity reached approximately 10% of that in water solution.