Determination of methylation site of DNA-methyltransferase NlaX by a hybrid methodстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M(.)NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pentanueleotide recognition sequence 5 =CCNGG-3' (where N = any nucleoside). 5-Methylcytosine (m(5)C) type modification by M.NlaX was confirmed by the use of oligonucleotide substrates that contain 5 fluoro-2 ' =deoxycytidine.