Degradation of proteinaceous substrates by xylotrophic basidiomycetesстатья
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Web of Science ,
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Dunaevsky Ya E. ,
Zhang D.,
Matveeva A.R.,
Belyakova G.A. ,
Belozersky M.A.
Журнал:
Microbiology
Том:
75
Номер:
1
Год издания:
2006
Издательство:
Pleiades Publishing, Ltd
Местоположение издательства:
Road Town, United Kingdom
Первая страница:
35
Последняя страница:
39
DOI:
10.1134/S0026261706010073
Аннотация:
The ability of various xylotrophs to produce extracellular proteolytic enzymes has been studied, with emphasis on medium-related factors regulating their secretion. Direct measurement of proteolytic activity in the culture liquid and postelectrophoresis determination of protease activity in polyacrylamide gel copolymerized with gelatin demonstrated that the secreted enzymes are quantitatively and qualitatively diverse. Activity levels of extracellular proteolytic enzymes strongly depend on pH and contents of protein and carbohydrate in the medium. All secreted proteases notably differed in molecular weight (of 51 kDa or higher and in excess of 95 kDa) and belonged mostly to two classes of proteolytic enzymes (serine proteases and metalloproteinases). © Pleiades Publishing, Inc., 2006.
Добавил в систему:
Белякова Галина Алексеевна