Visualization of Humic Acids Penetration in Higher Plants Vascular System Using Tritium Autoradiographyтезисы доклада

Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.

Работа с тезисами доклада

[1] Visualization of humic acids penetration in higher plants vascular system using tritium autoradiography / D. Abroskin, V. Korobkov, A. Beer et al. // Abstracts of Second International Conference of CIS IHSS on Humic Innovative Technologies Natural and engineered nanoparticles in clean water and soil technologies. — Vol. 2. — Moscow, Russia, 2012. — P. 3–3. Stimulatory effect of HA on the plant growth and germination is well known. Nevertheless, the mechanism of that effect is unknown, as well as the HA distribution in the plant tissues. Recently, a new method of direct labeling of humic substances with tritium was developed that allows producing labeled preparation completely identical in their properties to the native preparations [1] and an opportunity to use the produced humics to study their behavior in living cells of bacteria has been demonstrated [2]. The objective of the study was to estimate uptake of HA by higher plants and to obtain direct evidence of HA penetration in higher plants vascular system using tritium autoradiography. Coal HA was isolated from potassium humate produced from leonardite (H/C 0.87; O/C 0.5; C/N 53; Mn = 2.1). Seedlings of wheat Triticum aestivum L. were used for the experiments. Eight-day old plants were transferred into the vials containing 15 mL of HA at concentration 5 mg⋅L–1 with specific radioactivity 0.057 Curie⋅L–1. Three plants were used per one vial. Absorption time was 24 hours. All the experiments were performed in five replications. The HA distribution study in the plant was performed using tritium autoradiography. For the microautoradiography studies, the sections from wheat shoots and roots was excised and fixed in Navashin buffer according to [3]. Then plant samples were subjected to autoradiography. The nuclear emulsion was used for microautoradiography. Labeled samples getting and purification them from exchange position tritium were held under regulations of [1]. Obtained autoradiogram showed that HA intensely adsorbed on the root surface and accumulated primarily in the cortex area. HA absorbed by plants arrive in the central cylinder zone mainly from apoplast. In the central cylinders HA found in the xylem vessels, which indicate a possibility of acropetally transport. It is important to note that the using of nuclear emulsion allowed us to obtain a much better resolution. In this way the presence of HA in the cortex was shown, as well as within the xylem vessels. References. 1. Badun G.A., Chernysheva M.G., Tyasto Z.A., Kulikova N.A., Kudryavtsev A.V., Perminova I.V. A new technique for tritium labeling of humic substances. 2010. Radiochim. Acta, 98:161-166. 2. Kulikova N.A., Perminova I.V., Badun G.A., Chernysheva M.G., Koroleva O.V., Tsvetkova E.A. 2010. Estimation of uptake of humic substances from different sources by Escherichia coli cells under optimum and salt stress conditions by use of tritium-labeled humic materials. Appl. Environ. Microbiol. 2010, Vol. 76, No. 18, p. 6223-6230. 3. NS Voronin Guide to laboratory studies on the anatomy and morphology of plants. Moscow, Prosveschenie, 1981, 160s.

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