Interaction of Coal Humic Substances with White Rot Fungi: an Approach with Tritium Labeled Preparationsтезисы доклада

Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.

Работа с тезисами доклада

[1] Interaction of coal humic substances with white rot fungi: an approach with tritium labeled preparations / O. Klein, N. Kulikova, G. Badun et al. // Abstracts of Second International Conference of CIS IHSS on Humic Innovative Technologies Natural and engineered nanoparticles in clean water and soil technologies. — Vol. 2. — Moscow, Russia, 2012. — P. 23–23. Humic substances (HS) are defined as the products of complex transformation of organic residues with both biotic and abiotic factors being involved in this process. Among the biotic factors much attention has been paid recently to xylotrophic microorganisms with particular emphasis on white-rot fungi as they produce a wide spectrum of extracellular oxidoreductases providing HS transformation. However, interactions of white rot fungi with HS are poorly elucidated. In the current study uptake of HS by Basidiomycete was investigated under different carbon source availability (nutrition media with or without glucose were used). Experiments were performed using pure culture of Basidiomycete Trametes maxima 0275 (Wulf. Ex. Fr) Quel. and tritium labeled leonardite HS ([3H]HS). After 7 d of T. maxima submerged cultivation [3H]HS were added into the medium to final concentration 50 mg l–1 followed by subsequent cultivation for another 24 h. Performed experiments showed that HS were easily sorbed by fungi mycelium, and a significant portion of HS introduced could be found in the intracellular space of fungi. Amount of HS sorbed by fungi was estimated as 0.52±0.05 and 1.1±0.3 mg g–1 of fresh mycelium weight for the case of nutrition medium with and without glucose respectively. It should be noted also that along with increase of the amount of HS sorbed under carbon deficiency conditions the value of HS penetrated cells raised as well. When glucose was added, the value of HS penetrated the cells was accounted for 0.17±0.02 mg g–1 of fresh mycelium weight whereas in case with absence of glucose that value increased to 0.56±0.09 mg g–1. So, our experiments demonstrated that depending carbon source presence or absence the amount of HS penetrated value varied from 32 to 51% of humics totally taken up by fungi. The latter was evident for the fact that HS were seemingly to be utilized by fungi as an alternative carbon source.

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