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Depth distribution of bacteriochlorophyll from green sulphur bacteria in the Trekhtzvetnoe, Bolshie Khruslomeny lakes and the lagoon on the cape Zeleny in September 2018тезисы доклада Тезисы
- Авторы: Emelyantsev Pavel, Zhiltsova Anna, Kharitonov Dmitriy, Freidkin Mikhail, Krasnova Elena, Voronov Dmitriy, Svetlana Patsaeva
- Сборник: Abstract book of the International conference “Marine biology, geology and oceanography – interdisciplinary studies based on the marine stations and labs. 80th anniversary of the Nikolai Pertsov White Sea Biological Station.”
- Тезисы
- Год издания: 2018
- Издательство: KMK Scientific Press
- Местоположение издательства: Moscow, Russia (Federation)
- Первая страница: 64
- Последняя страница: 64
- Аннотация: The work is devoted to the development of a method for determining the concentration of bacteriochlorophylls (BChl) of green sulfur bacteria (Chlorobiaceae), phototrophs that live in the anaerobic zone of meromictic reservoirs. The new method which uses absorption spectroscopy without extracting pigments by organic solvents was developed to simplify the procedure of determining the BChl concentration. Green-colored and brown-colored cultures of GSB were selected as samples, as well as samples taken in March 2018 from three meromictic reservoirs. The samples from the same reservoirs taken in September 2018 were used to build depth profiles. Absorption spectra were measured using a Solar PV1251 spectrophotometer. The method involves determining the concentration of chlorosomal BChl over the area under the long-wave absorption peak in the absorption spectra of living microorganisms in water. A good linear correlation was obtained between the area under the longwavelength absorption peak in water and the concentration of BChl calculated using the Overman-Tilzer formula for extracts. From the linearity of the correlation graphs the formula for determining the concentration of BChl was obtained. Using the correlation coefficients obtained in March 2018 for the lagoon on cape Zeleny, Bolshie Khruslomeny and Trekhtzvetnoe lakes, concentrations of BChl were calculated for samples taken in September 2018. The depth distribution profiles of BChl concentrations were constructed for those three lakes.
- Добавил в систему: Мардашова Мария Валерьевна