|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
[Study of the structure and mechanism of action of troponin C and calmodulin by "protein" engineering]статья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Автор: Gusev N.B.
- Журнал: Biochemistry (Moscow)
- Том: 60
- Номер: 3
- Год издания: 1995
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 395
- Последняя страница: 418
- Аннотация: The structure, properties and action mechanisms of troponin C and calmodulin are reviewed. The primary and tertiary structures of calcium-binding proteins and the mechanisms of Ca2+ binding are analyzed. The methods used for investigating the functional activity of Ca-binding proteins are compared. Molecular biology approaches for analyzing the role of various ligands in Ca2+ binding are described. The role of alpha-helices in the maintenance of the overall structure, Ca2+ binding and calmodulin and troponin C interaction with target proteins is discussed. Mutations directed at the change of the electric charge and hydrophobicity of calmodulin and troponin C are described and compared. Data on the incorporation of Cys residues into calmodulin and troponin C structure are presented. The use of Cys-containing mutants of Ca-binding proteins for the study of conformational changes and protein-protein interaction is analyzed.
- Добавил в систему: Гусев Николай Борисович