|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
The functional effects of mutations Thr673–>Asp and Ser702–>Asp at the Pro-directed kinase phosphorylation sites in the C-terminus of chicken gizzard caldesmonстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Redwood C.S., Marston S.B., Gusev N.B.
- Журнал: FEBS Letters
- Том: 327
- Номер: 1
- Год издания: 1993
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 85
- Последняя страница: 89
- Аннотация: We expressed the C-terminal 99 amino acids of chicken gizzard caldesmon (658C) and two point mutants in which the preferred phosphorylation sites of MAP kinase and p34cdc2 kinase, Ser702 and Thr673 were altered to aspartic acid. The T673D mutant was indistinguishable from 658C but S702D was not phosphorylated by MAP kinase, was significantly less potent as an inhibitor of actin-tropomyosin activation of myosin MgATPase, and bound less actin-tropomyosin at low concentrations. Thus Ser702 is involved in the tropomyosin-dependent inhibitory mechanism of caldesmon, and its phosphorylation by MAP kinase or p34cdc2 kinase could modulate caldesmon function.
- Добавил в систему: Гусев Николай Борисович