Influence of Different Types of Block Copolymers on the Secondary Structure and Stability of the Staphylolytic Lysk Enzymeстатья
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Дата последнего поиска статьи во внешних источниках: 29 мая 2019 г.
Аннотация:A branched block copolymer of polyethyleneimine and polyethylene glycol (PEI-PEG) and a linearblock copolymer of polylysine and polyethylene glycol (PLL-PEG) form complexes with the staphylolyticLysK enzyme. The complexation between LysK and cationic block-copolymers results in a 1.5–2.0-foldincrease in the lytic activity of LysK and a 2–20-fold increase in its half-life. The stabilizing effects of theblock copolymers depend on the temperature, the NaCl concentration, and the polymer/enzyme ratio. Beingan effective stabilizer, linear PLL–PEG provides an increase in the LysK stability suitable for biomedicalmaterials (LysK maintains 100% of its activity after four months at 4°C).