Properties of the probiotic strain  Lactobacillus plantarum 8-RA-3 grown in a biofilm by solid substrate cultivation method

Ushakova, N.A.; Abramov, V.M.; Khlebnikov, V.S.; Semenov, A.M.; Kuznetsov, B.B.; Kozlova, A.A.; Nifatov, A.V.; Sakulin, V.K.; Kosarev, I.V.; Vasilenko, R.N.; Sukhacheva, M.V.; Melnikov, V.

Авторы: Ushakova N.A., Abramov V.M., Khlebnikov V.S., Semenov A.M., Kuznetsov B.B., Kozlova A.A., Nifatov A.V., Sakulin V.K., Kosarev I.V., Vasilenko R.N., Sukhacheva M.V., Melnikov V.
Журнал: Probiotics and Antimicrobial Proteins
Том: 4
Номер: 3
Год издания: 2012
Издательство: Springer Pub. Co.,
Местоположение издательства: United States
Первая страница: 180
Последняя страница: 186
DOI: 10.1007/s12602-012-9106-y
Аннотация: The biofilm formation took place in 48 h within the solid substrate cultivation of Lactobacillus plantarum 8-RA-3 strain on the wheat bran saturated with the MRS medium. The drying of the bran fermented by lactobacilli resulted in a decrease in the number of colony-forming units (CFU) from 23.0 × 108 to 6.9 × 105 CFU/g in daily samples and to less than 104 CFU/g in 2- and 3-day samples. However, according to the fluorescence-based live/dead assay data, more than 40 % of the non-cultured bacteria were viable. As a result of mice kept on a diet with the introduction of bran fermented by Lact. plantarum 8-RA-3 for 72 h into the fodder, a recovery of normal level of intestinal lactobacilli, inhibited by administration of antibiotic was noted. The strain genetically identical to the Lact. plantarum 8-RA-3 was isolated from the feces of these mice. The results indicate that solid substrate cultivated Lact. plantarum 8-RA-3 strain formed a biofilm. Once dried and transferred into a non-cultured state, biofilm cells retained its viability and biological activity.
Добавил в систему: Семенов Александр Михайлович

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Properties of the probiotic strain Lactobacillus plantarum 8-RA-3 grown in a biofilm by solid substrate cultivation methodстатья

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