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Basic guidelines for the selection of polyelectrolytes that can effectively prevent thermal aggregation of enzymes without any substantial loss in their catalytic activityстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Stogov S.V.a, Muronets V.I.a, Izumrudov V.A.b
- Журнал: Polymer Science - Series C
- Том: 53
- Номер: 1
- Год издания: 2011
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 97
- Последняя страница: 106
- DOI: 10.1134/S1811238211030027
- Аннотация: Factors governing thermal aggregation and thermal denaturation of a model homotetrametric enzyme, glyceraldehyde-3-phosphate dehydrogenase, electrostatically bound to a polyelectrolyte are revealed. Polyanions with sulfate or sulfonate groups in chains are the most efficient suppressors of aggregation. However, their presence promotes thermal denaturation. Short chains of relatively hydrophobic polyanions exert a strong denaturing effect that changes the secondary, tertiary, and quaternary structures of a protein. Basic guidelines for the selection of a polyelectrolyte are outlined. According to the proposed guidelines, hydrophilic high-molecular-mass macromolecules able to form stable soluble polyelectrolyte complexes with the enzyme are the most efficient at preventing thermal aggregation without substantial loss in catalytic activity. © Pleiades Publishing, Ltd., 2011.
- Добавил в систему: Изумрудов Владимир Алексеевич