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Determination of aflatoxin B1 in rice, barley, and feed by non-instrumental immunochromatographic strip-test and high sensitive ELISAстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Shim Won-Bo, Kim Jung-Sook, Kim Ji-Young, Choi Jin-Gil, Jung-Hyun Je, Kuzmina Nina Sergeevna, Eremin Sergei Alexandrovich, Chung Duck-Hwa
- Журнал: Food Science and Biotechnology
- Том: 17
- Номер: 3
- Год издания: 2008
- Издательство: Korean Society of Food Science and Technology
- Местоположение издательства: Korea, Republic of
- Первая страница: 623
- Последняя страница: 630
- Аннотация: A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DGELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both inethods were coincided with the amount spiked AFB 1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the lCG strip-test is easier to perform and quicker, but less sensitivity than DGELISA. Both inethods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the lCG strip-test call be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.
- Добавил в систему: Еремин Сергей Александрович