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Determination of Alachlor in food products by a fluorescence polarization immunoassayстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Nartova Yu V., Ermolaeva T.N., Fleisher M.R., Abuknesha R., Eremin S.A.
- Журнал: Journal of Analytical Chemistry
- Том: 63
- Номер: 5
- Год издания: 2008
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 499
- Последняя страница: 505
- DOI: 10.1134/S106193480805016X
- Аннотация: A procedure for the determination of Alachlor in liquid media using a fluorescence polarization immunoassay (FPIA) was developed. The effects of the structure and purification degree of a tracer (analyte with a fluorescent label) on the analytical signals, sensitivity, and selectivity in the determination of Alachlor were studied. The tracer-antibody binding constants (K-T) and antigen-antibody binding constants (affinity constants K-Af) were calculated, and optimum immunoreagent pairs for the determination of Alachlor were chosen. The calibration graph was linear over the Alachlor concentration range 0.01-1.0 mu g/mL; the detection limit was 8 ng/mL. The procedure was tested in the analysis of model solutions and soy-containing food products (sausages).
- Добавил в систему: Еремин Сергей Александрович