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Monoclonal-based enzyme-linked immunosorbent assay for the detection of zearalenone in cerealsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Thongrussamee T., Kuzmina N.S., Shim W.B., Jiratpong T., Eremin S.A., Intrasook J., Chung D.H.
- Журнал: Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment
- Том: 25
- Номер: 8
- Год издания: 2008
- Издательство: Taylor and Francis
- Местоположение издательства: United States
- Первая страница: 997
- Последняя страница: 1006
- DOI: 10.1080/02652030701823159
- Аннотация: A monoclonal antibody against zearalenone (ZEA) was produced and used successfully to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for the analysis of ZEA in cereals. This DC-ELISA had a limit of detection of 0.15 +/- 0.02 mu g 1(-1) and an IC(50) value of 1.13 +/- 0.16 mu g 1(-1). Matrix interference was minimized by dilution of the sample extract before ELISA assays. Aqueous methanol (80%) gave good extraction efficiencies, and the recovery from spiked rice, barley, and corn samples averaged between 87 and 112%. Although ZEA was detected in seven (9%) of 80 rice samples and in eight (16%) of 50 barley samples, the concentration of ZEA in samples was around or below the limit of detection of DC-ELISA. Among 38 corn samples, ZEA was detected in nine (24%) samples in the range 41.0-909.8 mu g kg(-1). Re-analysis of the ELISA-positive corn samples by high-performance liquid chromatography (HPLC) confirmed that seven (18%) corn samples were positive. The ZEA results for corn showed very good agreement between DC-ELISA and a commercial AgraQant(R) zearalenone kit (r(2) = 0.98). Thus, the monoclonal antibody-based DC-ELISA could be applied to the preliminary screening of ZEA contamination when analysis of a large sample number is needed.
- Добавил в систему: Еремин Сергей Александрович