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Multi-Wavelength Thermal-Lens Spectrometry for High-Accuracy Measurements of Absorptivities and Quantum Yield of Photodegradation of a Hemoprotein-Lipid Complexстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 октября 2017 г.
- Авторы: Tishchenko K., Muratova M., Volkov D., Filichkina V., Nedosekin D., Zharov V., Proskurnin M.
- Журнал: Arabian Journal of Chemistry
- Том: 10
- Номер: 6
- Год издания: 2017
- Издательство: Elsevier Science
- Местоположение издательства: United Kingdom
- Первая страница: 781
- Последняя страница: 791
- DOI: 10.1016/j.arabjc.2016.01.009
- Аннотация: Accurate measurements of absorptivities and quantum yields of biochemical species under various conditions are an important task of applied photonics and analytical chemistry. In this work, we provide a comparison of the capabilities of thermal-lens spectrometry to measure these parameters of various samples. Measurements of relevant model substances, biologically active substituted 2-thiohydantoins and their complexes of copper(I,II) and heme proteins (forms of hemoglobin and cytochrome c), showed negligible differences in apparent molar absorptivities for thermal-lens spectrometry and optical-absorption (spectrophotometric) data. The values for tabletop and microscale thermal-lens measurements under batch conditions differ insignificantly. The precision of measurements of molar absorptivities by thermal-lens spectrometry is no less than in the case of spectrophotometry or the precision is even higher in the cases of low absorptivities. For cardiolipin–cytochrome c-NO complex, the difference between absorptivity values calculated from thermal-lens data and acquired by spectrophotometry is significant due to complex photodegradation. The quantum yield of its photolysis reaction calculated from optical absorption and thermal-lens data altogether at two wavelengths for 0.4–360 s of the reaction, 0.46 ± 0.04, was estimated. Keywords Multispectral photothermal spectroscopy; Thermal-lens spectrometry; 2-thiohydantoins (2-thioxo-imidazolidin-4-ones); Cytochrome c; Hemoglobin; Absorptivity
- Добавил в систему: Волков Дмитрий Сергеевич