Investigation of the activity and stability of papain in different micellar systemsстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 февраля 2014 г.
Авторы:
Filatova L.Y. ,
Oxenoyt Y.S. ,
Yakovleva K.S. ,
Ivanova N.I. ,
Klyachko N.L.
Журнал:
Moscow University Chemistry Bulletin
Том:
65
Номер:
2
Год издания:
2010
Издательство:
Allerton Press Inc.
Местоположение издательства:
United States
Первая страница:
80
Последняя страница:
86
DOI:
10.3103/S0027131410020057
Аннотация:
Hydrolysis of PyrPheAlaPNa, catalyzed by papain in reverse micelles of ionogenic surfactant of Aerosol OT, and in octane, was studied in the presence and in the absence of nonionogenic surfactants of a different nature. As found, the dependence of the catalytic activity of papain on the hydration degree of the surfactant in reverse micelles of AOT has two maxima, corresponding to the activity of monomeric and oligomeric forms of the enzyme. It was established that the addition of nonionogenic surfactant of a different nature to the Aerosol OT in octane leads to changes of the catalytic properties of the enzyme, in particular, to its activation in the presence of Pluronic L61. Nanoemulsions of nontoxic nonionogenic surfactant and organic solvents, lecithin and Tween 20 in eucalyptus oil were created and characterized; with the use of the photon-correlation spectroscopy technique it was shown that the size of such particles lie in the range from 34 to 266 nm, depending on the water content, nature, and the concentration of system components, and factors regarding its shape lie in the interval 20-30, suggesting the presence of particles with a rod-like shape. It is important to note that in such systems, suitable for use in medicine and cosmetology, the activity of papain was revealed. В© 2010 Allerton Press, Inc.
Добавил в систему:
Клячко Наталья Львовна