|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
A new gene encoding the ribosome-inactivating protein from mistletoe extractsстатья
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Tonevitsky AG, Agapov II, Pevzner IB, Maluchenko NV, Moisenovich MM, Palmer RA, Yurkova M., Pfüller K., Pfüller U.
- Журнал: Progress in drug research. Fortschritte der Arzneimittelforschung. Progr∎s des recherches pharmaceutiques
- Том: 54
- Номер: 4
- Год издания: 2004
- Издательство: Birkh∎user Verlag.
- Местоположение издательства: Basel, Switzerland
- Первая страница: 242
- Последняя страница: 249
- DOI: 10.1055/s-0031-1296966
- Аннотация: Extracts from mistletoe (Viscum album L.) contain three main toxic proteins -the lectins MLI (also known as vls-cumln), MLII and MLIII. A catalytic subu-nlt of the mistletoe plant toxic lectin MLIII has been cloned and expressed In Escherichia coli cells. The structure and immunochemical properties of recombinant MLIII A-subunlt were Investigated using a panel of monoclonal antibodies against ML-toxlns. Rlbosome-lnactlvating activity of the recombinant MLIII A-subunlt was determined In a cell-free system exhibiting Inhibition of endogenous protein synthesis. The comparative analysis of nucleotide and deduced amino acid sequences of the cloned MLIII A and the native MLI A-subunlts was performed, revealing the main differences In the primary structure of these proteins. Antigenicity analysis of the MLIII A-subunlt has revealed a new epitope d179-E184 that is not present in viscumln. The role of toxic lectins with respect to the immunological properties of mistletoe extracts Is discussed.
- Добавил в систему: Малюченко Наталия Валериевна