Inhibition of mitochondrial bioenergetics: the effects on structure of mitochondria in the cell and on apoptosis

Lyamzaev, K.G.; Izyumov, D.S.; Avetisyan, A.V.; Yang, F.; Pletjushkina, O.Y.; Chernyak, B.V.

Авторы: Lyamzaev K.G., Izyumov D.S., Avetisyan A.V., Yang F., Pletjushkina O.Y., Chernyak B.V.
Журнал: Acta Biochimica Polonica
Том: 51
Номер: 2
Год издания: 2004
Издательство: Polskie Towarzystwo Biochemiczne
Местоположение издательства: Poland
Первая страница: 553
Последняя страница: 562
Аннотация: The effects of specific inhibitors of respiratory chain, F(o)F(1)ATP synthase and uncouplers of oxidative phosphorylation on survival of carcinoma HeLa cells and on the structure of mitochondria in the cells were studied. The inhibitors of respiration (piericidin, antimycin, myxothiazol), the F(1)-component of ATP synthase (aurovertin) and uncouplers (DNP, FCCP) did not affect viability of HeLa cells, apoptosis induced by TNF or staurosporin and the anti-apoptotic action of Bcl-2. Apoptosis was induced by combined action of respiratory inhibitors and uncouplers indicating possible pro-apoptotic action of reactive oxygen species (ROS) generated by mitochondria. Short-term incubation of HeLa cells with the mitochondrial inhibitors and 2-deoxyglucose followed by 24-48 h recovery resulted in massive apoptosis. Apoptosis correlated to transient (3-4 h) and limited (60-70%) depletion of ATP. More prolonged or more complete transient ATP depletion induced pronounced necrosis. The inhibitors of respiration and uncouplers caused fragmentation of tubular mitochondria and formation of small round bodies followed by swelling. These transitions were not accompanied with release of cytochrome c into the cytosol and were fully reversible. The combined effect of respiratory inhibitors and uncouplers developed more rapidly indicating possible involvement of ROS generated by mitochondria. More prolonged (48-72 h) incubation with this combination of inhibitors caused clustering and degradation of mitochondria.
Добавил в систему: Черняк Борис Викторович

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ИНХС РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ИНХС РАН

Inhibition of mitochondrial bioenergetics: the effects on structure of mitochondria in the cell and on apoptosisстатья

Прикрепленные файлы