P.7.003 Acute hypoxia, modelled in early pregnancy, causes transgenerational effects on child neurodevelopmentстатьяТезисы
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
Аннотация:Introduction.: Prenatal stress is one of the main reasons of the
phenomenon of intrauterine programming, which shows its effects
even in adulthood. It is well proved, that negative influence of
different pathologic factors during pregnancy is not limited by one
generation. However the consequences of acute stress happened in
early period of gestation are rarely investigated. So the aim of the
present study was to evaluate acute hypobaric hypoxia effects on
pregnant rats (generation F0) as well as on their direct descendants
(generation F1) and descendants resulted by interbreeding females
from generation F1 with intact males (generation F2).
Methods: The white rats on the 9−10th days of gestation were
subjected to acute hypobaric hypoxia in an altitude chamber.
The first day of pregnancy was determined by the presence of
spermatozoa in the vaginal smear. Control pregnant females were
not subjected to acute hypobaric hypoxia. On the 2nd day after
the delivery pups were weighed, and from the 3rd till the 8th day
post partum maternal behavior in the home cage was observed. A
modified ‘snap-shot’ method of Myers has been used: every rat
has been observed for 5 seconds every 5 minutes. A number of
variables such as mother being in or out of nest, being in contact
with pups, licking/grooming of pups, nursing, etc. has been
recorded. After growing up the female posterity (F1 dams) were
mated with intact males, and their maternal behavior was observed
in the same way. Two posterity generations has been grown up.
The estimation of physical development in pups was carried out
from the day of birth up to the 57th day of life. At the age of 22
(critical age for survival because of the end of weaning and switch
to adult nutrition type) and 57 days (considered to be pubertal
period) the motor and exploratory activity of experimental animals
were tested in “Hole board” while the anxiety level was assessed
using an elevated plus-maze. The cognitive function was tested in
complex maze with positive reinforcement.
Results: It was shown that pregnant rats survived hypoxia (F0)
demonstrated the increased maternal behavior (more contact with
pups and total nursing then control dams) while females from
generation F1 showed its decrease (more leavings of nest, less
contact with pups and less total nursing). Despite of oppositely
directed modifications of maternal behavior, demonstrated by F0
and F1 females, F1 and F2 posterity showed similar deviations of
physical development and behavioral. Acute hypobaric hypoxia
led to the delay in eyes opening and the decrease of height
and weight in posterity of F1 and F2 generations, compared to
control animals. Besides males and females of both generations
demonstrated the decreased linear locomotion and exploratory
activity following with raising in anxiety level. At the same time
the learning capacity was improved in females from F1 generation
and was reduced in F2 males.
Conclusion: Data obtained support the great role of early
organogenesis for development of not only direct posterity but
also following generations.