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Peptide synthesis in dimethylformamide-based organic media catalysed by non-covalent chymotrypsin-polyelectrolyte complexesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Vakurov A.V.a, Gladilin A.K.a, Partridge J.b., Izumrudov V.A.a, Mozhaev V.V.a.b.c, Levashov A.V.a, Halling P.J.b.d
- Журнал: Biocatalysis and Biotransformation
- Том: 17
- Номер: 4
- Год издания: 1999
- Издательство: Taylor & Francis
- Местоположение издательства: United Kingdom
- Первая страница: 283
- Последняя страница: 291
- Аннотация: Chymotrypsin-catalysed kinetically-controlled peptide synthesis was studied in mixtures of dimethylformamide (DMF) and acetonitrile containing 6% water. The free enzyme showed no detectable activity with 60% or more DMF. However, by pre-forming a complex with polyacrylate (optimum 50 carboxyl groups to 1 enzyme molecule), activity and stability were good at 60% DMF, and reasonable at 70% DMF. In 60% DMF, the peptide Ac-Tyr-Lys-NH2 could be made in 95% yield using this catalyst. Chymotrypsin-catalyzed kinetically-controlled peptide synthesis was studied in mixtures of dimethylformamide (DMF) and acetonitrile containing 6% water. The free enzyme showed no detectable activity with 60% or more DMF. However, by pre-forming a complex with poly-acrylate (optimum 50 carboxyl groups to 1 enzyme molecule), activity and stability were good at 60% DMF, and reasonable at 70% DMF. In 60% DMF, the peptide Ac-Tyr-Lys-NH2 could be made in 95% yield using this catalyst.
- Добавил в систему: Изумрудов Владимир Алексеевич