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Study of interaction of N,N,N',N'-tetramethyl-p-phenylenediamine with chloroplast membranes under the action of lightстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Bulychev A.A., Kurella G.A., Lukashev E.P.
- Журнал: Biochemistry (Moscow)
- Том: 48
- Номер: 4
- Год издания: 1983
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 571
- Последняя страница: 576
- Аннотация: The light-induced changes in the external concentration of the oxidized form of the redox mediator, tetramethyl-p-phenylenediamine (TMPD+) were recorded in suspensions of pea chloroplasts using an ion-selective electrode. A comparison of the photoinduced changes in the TMPD+ concentration, the pH, the optical density of the suspension at 560 nm, and the dynamics of oxygen exchange showed that in the initial period of illumination in the absence of reducing agents TMPD+ acts as an electron acceptor and the begins to function as a carrier of electrons between photosystems II and I. The dark reoxidation of TMPD in cyanide-treated and untreated chloroplasts after brief exposures to light takes place slowly, with a rate constant of about 0.01 s-1. The dark increase in TMPD+ concentration in the absence of inhibitors after illumination for 30-60 s takes place relatively rapidly, with a rate constant of 0.1 s-1. It is assumed that TMPD is reduced at the beginning of the illumination (5-10 s), but later is partically reoxidized and the cation TMPD+ is accumulated in the thylakoids.
- Добавил в систему: Булычев Александр Александрович