Аннотация:Accuracy of spot detection and quantification plays a critical role in the analysis of ELISPOT data.
Differences in staining intensities of spots and their morphological variations make it difficult developing
a reliable software application. We have developed an image recognition method allowing for the automatic
detection of round objects (spots) on ELISPOT images independently of the registration conditions.
The emphasis is done on objects of elliptical shape which is typical for a wide range of spots that can be
analyzed by both monochrome and a dual-color version of our software. The method of subdivision of
objects into groups is also described which is based on color attributes of spots.