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[ANSA analysis. I. Mixtures of substrates in analyzing proteases: features of the kinetics of hydrolysis during competition]статья
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Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Nedospasov A.A., Lifanov A.P., Rodina E.V.
- Журнал: Biochemistry (Moscow)
- Том: 59
- Номер: 10
- Год издания: 1994
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1511
- Последняя страница: 1520
- Аннотация: Studies designed to investigate the utility of mixtures of chromogenic peptide substrates for protease analysis are reviewed. Individual applications of aminonaphthalenesulfonamide substrates with a variable structure of the leaving group are discussed. The kinetic curves describing the mixtures of competing substrates are presented and the conditions of the occurrence of S-shaped curves specified. The kinetic differences for mixtures of competing substrates split by a single enzyme and for those independently split by various substrates are demonstrated. A comparison between experimental and calculated kinetic curves is made. The cause of erratic results obtained in a number of studies using substrate mixtures is indicated.
- Добавил в систему: Родина Елена Валерьевна