Flux balance analysis of photoheterotrophic growth of purple nonsulfur bacteria relevant to biohydrogen productionстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Purple nonsulfur bacteria can produce hydrogen during photoheterotrophic growth on
a variety of organic substrates. To facilitate metabolic engineering of this group of bacteria
with the purpose of maximizing hydrogen production, we have developed the first
comprehensive mathematical model of anaerobic photoheterotrophic metabolism. This
model is a stoichiometric model to which Flux Balance Analysis also known as constraintbased
analysis has been applied. Because Rhodobacter sphaeroides emerged as one of the
best understood purple nonsulfur bacteria, we based our modeling on this species.
However, the approach is readily applicable to other purple nonsulfur bacteria. The
metabolic space used in model construction contains 314 reconstructed biochemical
reactions and 287 compounds. The list of simulated metabolic pathways involves major
carbon and nitrogen metabolic pathways. A distinctive feature of our model is that it
considers maximization of two different cell objective functions, namely, biomass
production rate, which is commonly used in flux balance models, and reductant removal
rate, which is a unique feature of this model reflecting peculiarity of photoheterotrophic
metabolism. The performance of this model has been assessed by comparing computed
metabolic fluxes with experimental values obtained by several research groups who
worked on various strains of purple nonsulfur bacteria and utilized different growth
setups. The model testing revealed remarkably good agreement between the predicted and
measured metabolic fluxes, which validates the presented model.