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Three‐photon‐resonance‐enhanced third‐harmonic generation for label‐free deep‐brain imaging: In search of a chemical contrastстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 января 2020 г.
- Авторы: Lanin A.A., Chebotarev A.S., Pochechuev M.S., Kelmanson I.V., Fedotov A.B., Belousov V.V., Zheltikov A.M.
- Журнал: Journal of Raman Spectroscopy
- Том: 50
- Год издания: 2019
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 1296
- DOI: 10.1002/jrs.5566
- Аннотация: Within the past decade, nonlinear Raman microscopy has earned a well‐deserved status of a gold‐standard technology for chemically selective imaging. Even though second‐ and third‐harmonic microscopy is much less demanding on a laser source and multifrequency beam arrangement, it is increasingly falling behind nonlinear Raman scattering as a method of bioimaging because it offers no mechanism whereby imaging could be made chemically specific. Here, we show, however, that such a mechanism does exist, helping harmonic‐generation microscopy overcome its no‐chemical‐specificity handicap. We demonstrate that, with the laser wavelength tuned to a three‐photon resonance with the Soret band of hemoglobin, third‐harmonic generation provides a chemically specific method for a high‐contrast imaging of red blood cells in a broad class of biological systems, including live brain. Moreover, third‐harmonic generation imaging can be conveniently combined with second‐harmonic microscopy on a compact laser platform, providing, as our experiments on rat brain show, a powerful resource for three‐dimensional, cell‐specific label‐free deep‐brain imaging.
- Добавил в систему: Желтиков Алексей Михайлович
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