Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomesстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Messbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6863 devoid of photosystem I grown in a Fe-57-containing medium were measured. The spectrum is a broadened dublet whose. size (about 20%) and parameters (isomeric shift delta = 0.3 mm/s and quadrupole splitting Delta = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic. state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.