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ACIDIC INACTIVATION AND CA-DEPENDENT REACTIVATION OF THE CHLOROPLAST ELECTRON-TRANSFER CHAINстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: SEMIN BK, CHURKINA LA, KONEV YN, SOLNTSEV MK, VASILEV IR, IVANOV II
- Журнал: Molecular Biology
- Том: 28
- Номер: 3
- Год издания: 1994
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 344
- Последняя страница: 348
- Аннотация: Incubation of pea (Pisum sativum L.) chloroplasts in citrate buffer of pH 3.3 leads to inhibition of electron transfer, which is restituted at pH 7.6 in the presence of Ca2+ but not Mg2+, Mn2+, or Ba2+ ions. The extent of inactivation and reactivation varies with different accepters: the activity declines to 27% of the initial with dichlorophenol-indophenol, to 62% with ferricyanide, and 16% with phenyl-p-benzoquinone. Donor-acceptor analysis and thermoluminescence assays suggest that Ca-depleted chloroplasts are inhibited in the acceptor part of photosystem II. Reactivation is suppressed by a water-soluble carbodiimide EDC but not by a lipid-soluble DCCD. Phenylbenzoquinone inhibits ferricyanide reduction in Ca-depleted chloroplasts but not in reactivated ones. The possibility of calcium control of the Q(B) binding site is discussed.
- Добавил в систему: Сёмин Борис Константинович