Comparative analysis of synaptonemal complexes in hybrid males of Caucasian pine voles (Rodentia, Microtinae, Terricola) related to studying hybrid sterilityстатья
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Аннотация:The synaptonemal complexes (SC) in two hybrid males of Caucasian pine voles obtained as a result of crossing karyotypically different pine vole species were studied using an electronic microscope. Two Robertsonian trivalents, sixteen bivalents, and univalents of sex chromosomes were presented in pachytene cells of hybrids (2n = 40, NF = 58) from crossing a T. daghestanicus 38-chromosome female with a 42-chromosome male (karyoform "A"). These trivalents correspond to the two meta- and four acrocentrics involved in Robertsonian fusion. No synaptic anomalies were observed in forming trivalents. Twenty-six autosome bivalents and sex chromosome univalents were detected in pachytene cells of male hybrids from crossing a hybrid female (T. majori female (2n = 54, NF = 60) x T, daghestanicus male (2n = 54, NF = 58) with a T, daghestanicus 54-chromosome male (NF = 58). Disruptions of synapsis in some bivalents were revealed in 6 out of 15 spermatocytes. These disruptions appear to indicate a more considerable isolation of T, daghestanicus from T. majori species than in the case of two T. daghestanicus karyoforms (2n = 38 and 2n = 42 "A"). A particular behavior of X and Y chromosome univalents was found in cells of the hybrid meiotic phase.