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Universal and rapid method for purification of GFP-like proteins by the ethanol extractionстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 мая 2019 г.
- Авторы: Samarkina O.N., Popova A.G., Gvozdik E.Y., Chkalina A.V., Zvyagin I.V., Rylova Y.V., Rudenko N.V., Lusta K.A., Kelmanson I.V., Gorokhovatsky A.Y., Vinokurov L.M.
- Журнал: Protein Expression and Purification
- Том: 65
- Номер: 1
- Год издания: 2009
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 108
- Последняя страница: 113
- DOI: 10.1016/j.pep.2008.11.008.
- Аннотация: GFP-like fluorescent proteins (FPs) are crucial in biological and biomedical studies. The majority of FP purification techniques either include multiple time-consuming chromatography steps with a low yield of the desired product or require prior protein modification (addition of special tags). In the present work, we propose an alternative ethanol extraction-based technique previously used for GFP purification and then modified for diverse FPs originated from different sources. The following recombinant FPs were expressed using Escherichia coli M15 (pREP4) strain as a host transformed with pQE30 plasmid bearing one of the target FP genes: TagCFP, TagGFP, TagYFP, TagRFP, TurboGFP, TurboRFP, Dendra2, TurboFP602 and KillerRed. Despite their diversity, all tested recombinant FPs were successfully purified and yielded a highly homogeneous product. The method is easily scalable for purification of any amount of protein and requires no expensive reagents and equipment.
- Добавил в систему: Рудимова Юлия Владимировна