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CHEMILUMINESCENT MICROPLATE-BASED ASSAYS FOR DETECTION OF NUCLEIC ACIDSтезисы доклада
- Автор: Сахаров И.Ю.
- Сборник: 3RD International Conference „Smart Bio“
- Тезисы
- Год издания: 2019
- Место издания: Kaunas Technological University Kaunas
- Первая страница: 32
- Последняя страница: 32
- Аннотация: Detection of DNA sequences is of great importance for cancer diagnostics, pathogen determination and forensic analysis. DNA detection techniques are often based on hybridization reaction. Although homogeneous methods are more rapid and simple, heterogeneous methods are more sensitive and less prone to matrix effect in real samples analysis. In our work some formats of microplate sandwich assay for DNA detection with different capture and reporter conjugates have been developed. DNA sequence of hepatitis B virus was used as a model target. Since the surface of microplate wells is limited, the highly sensitive detection system based on HRP-catalysed chemiluminescence with the use of phenothiazine enhancers was applied. This work was supported by the Russian Science Foundation (Grant No. 17-14-01042).
- Добавил в систему: Сахаров Иван Юрьевич