Interleukin-11 binds specific EF-hand proteins via their conserved structural motifsстатья
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Дата последнего поиска статьи во внешних источниках: 28 октября 2016 г.
Авторы:
Kazakov A.S. ,
Sokolov A.S.,
Vologzhannikova A.A. ,
Permyakova M.E. ,
Khorn P.A. ,
Ismailov R.G.,
Denessiouk K.A. ,
Denesyuk A.I. ,
Rastrygina V.A. ,
Baksheeva V.E. ,
Zernii E.Y. ,
Zinchenko D.V. ,
Glazatov V.V. ,
Uversky V.N. ,
Mirzabekov T.A. ,
Permyakov E.A. ,
Permyakov S.E.
Журнал:
Journal of Biomolecular Structure and Dynamics
Год издания:
2016
Издательство:
Adenine Press
Местоположение издательства:
United States
Первая страница:
1
Последняя страница:
14
DOI:
10.1080/07391102.2015.1132392
Аннотация:
Interleukin-11 (IL-11) is a hematopoietic cytokine engaged in numerous biological processes and validated as a target for treatment of various cancers. IL-11 contains intrinsically disordered regions that might recognize multiple targets. Recently we found that aside from IL-11RA and gp130 receptors, IL-11 interacts with calcium sensor protein S100P. Strict calcium dependence of this interaction suggests a possibility of IL-11 interaction with other calcium sensor proteins. Here we probed specificity of IL-11 to calcium-binding proteins of various types: calcium sensors of the EF-hand family (calmodulin, S100B and neuronal calcium sensors: recoverin, NCS-1, GCAP-1, GCAP-2), calcium buffers of the EF-hand family (S100G, oncomodulin), and a non-EF-hand calcium buffer (α-lactalbumin). A specific subset of the calcium sensor proteins (calmodulin, S100B, NCS-1, GCAP-1/2) exhibits metal-dependent binding of IL-11 with dissociation constants of 1–19 μM. These proteins share several amino acid residues belonging to conservative structural motifs of the EF-hand proteins, ‘black’ and ‘gray’ clusters. Replacements of the respective S100P residues by alanine drastically decrease its affinity to IL-11, suggesting their involvement into the association process. Secondary structure and accessibility of the hinge region of the EF-hand proteins studied are predicted to control specificity and selectivity of their binding to IL-11. The IL-11 interaction with the EF-hand proteins is expected to occur under numerous pathological conditions, accompanied by disintegration of plasma membrane and efflux of cellular components into the extracellular milieu. © 2016 Taylor & Francis
Добавил в систему:
Зерний Евгений Юрьевич