Immobilised cells of Pachysolen tannophilus yeast for ethanol production from crude glycerol

Stepanov, N.; Efremenko, E.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 января 2017 г.

Авторы: Stepanov N., Efremenko E.
Журнал: New Biotechnology
Том: 34
Год издания: 2017
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 54
Последняя страница: 58
DOI: 10.1016/j.nbt.2016.05.002
Аннотация: Screening among naturally occurring yeast strains of Pachysolen spp. that are capable of producing ethanol from glycerol under aerobic conditions identified the most active culture, P. tannophilus Y −475. Conversion of glycerol by this producer immobilised in poly(vinyl alcohol) cryogel resulted in a 90% yield of ethanol relative to the theoretical limit. The maximum rate of alcohol accumulation was 0.64 ± 0.01 g L−1 h−1 at a 25 g L−1 concentration of glycerol in the culture medium. We demonstrated the efficacy of reusing immobilised cells (for a minimum of 16 working cycles for batch mode of crude glycerol conversion to ethanol) and the possibility of long-term (for a minimum of 140 h) use of the cells in continuous mode with a maximum process productivity of 0.63 ± 0.02 g L−1 h−1, while the medium dilution rate in the reactor was 0.062 ± 0.001 h−1. Reduction of metabolic activity did not exceed 5□7% relative to baseline. Immobilised cells were demonstrated to withstand long-term storage in frozen form for at least 2 years while retaining high metabolic activity.
Добавил в систему: Ефременко Елена Николаевна

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Immobilised cells of Pachysolen tannophilus yeast for ethanol production from crude glycerolстатья

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