First report of Ilyonectria crassa on potato

Chudinova, E.; Platonov, V.; Elansky, S.; Alexandrova, A.; Kokaeva, L.; Krutyakov, Y.

Авторы: Elena Chudinova, Vladislav Platonov, Sergey Elansky, Alina Alexandrova, Lyudmila Kokaeva, Yurii Krutyakov
Журнал: Journal of Plant Pathology
Том: 101
Номер: 4
Год издания: 2019
Издательство: Societa Italiana di Patologia Vegetale
Местоположение издательства: Italy
Первая страница: 1293
Последняя страница: 1294
DOI: 10.1007/s42161-019-00355-x
Аннотация: Seed potato tubers were grown in a commercial field in Kostroma region of Russia. After harvest, affected tubers with small round dark depressions (usually around germs or wounds in tuber skin) were collected from the potato storage facility in October, 2017. Fungal mycelium from affected parts were transferred onto the surface of 1,5% malt agar medium amended with penicillin (1000 U/ml benzylpenicillin sodium). On the surface of the media the fungi formed a colony with white flaky mycelium. The medium beneath the mycelium turned reddish-brown. The fungus formed two spore types on conidiophores produced singly and aggregated into small sporodochia. Macroconidia were elongated, cylindrical, with one to three septa, the average length was 27.2 (23.2-32.2) µm, width – up to 4.9 µm. The average length of microconidia was 14.3 (10.3-18.1) µm, width up to 4.0 µm. All tested features showed affiliation of the strain to the species Ilyonectria crassa (Wollenw.) A.Cabral & Crous (anamorph Cylindrocarpon radicicola var. crassum Wollenw.) (Cabral et al., 2012). To confirm the species diagnosis, the structure of species-specific DNA loci were studied: region ITS1-5,8S-ITS2 (primers ITS5 / ITS4), fragments of β-tubulin (Bt2a / Bt2b) and TEF 1 (EF1-728F / EF1-986R) genes. DNA isolation, extraction of amplicons, sequencing and data processing were performed as described in Kutuzova et al., 2017. The sequences of nucleotides of the site ITS1-5,8S-ITS2 (NCBI-GenBank Accession MH818326), fragments of beta-tubulin (MH822872), and TEF 1α genes (MK281307) showed high identity to the corresponding accessions from the ex-lectotype of I. crassa, strain CBS 139.30 (JF735275 (100%), JF735393 (99,7%), JF735723 (99,3%)), and strain CBS 158.31 (ITS-no data, JF735394 (100%), JF735724 (99,3%)). Slices of potato tubers were inoculated with the drop (25 µl, 1,5 mm in diameter) of macroconidia suspension (105 pieces / ml). On the fifth day the lesion diameter reached 25 mm. The fungus was re-isolated from the mycelium developing on a potato tuber fragment. The isolate was completely identical to the parent strain. Ilyonectria crassa did not infect the whole intact potato tuber. Apparently, I. crassa is a wound parasite and can infect small sprouts in eyes too. According to our information, this is the first report of I. crassa on potato.
Добавил в систему: Еланский Сергей Николаевич

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