Determination of amino acid enantiomers in pharmaceuticals by reversed-phase high-performance liquid chromatography

Авторы: Chernobrovkin M.G., Anan’eva I.A., Shapovalova E.N., Shpigun O.A.
Журнал: Zhurnal Analiticheskoj Khimii
Том: 59
Номер: 1
Год издания: 2004
Первая страница: 55
Последняя страница: 63
DOI: 10.1023/B:JANC.0000011669.08932.d8
Аннотация: Precolumn derivatization with the reagent o-phthalic aldehyde/N-acetyl-L-cysteine (OPA/NAC) was used for the determination of amino acid enantiomers by reversed-phase high-performance liquid chromatography. The influence of the composition and pH of the eluent on the separation of the resulting derivatives was studied with the example of four amino acids. It was found that the highest selectivity and efficiency of the separation of OPA/NAC derivatives of amino acids is attained with the use of the eluent methanol–0.01 M Na2HPO4 (pH 6.0). The optimum composition of the mobile phase and conditions of the gradient elution were selected for the separation of a mixture of 20 amino acid derivatives. A procedure was developed for the determination of amino acid enantiomers in parenteral nutrition preparations. The procedure was used for the determination of D-isomers of arginine, alanine, methionine, phenylalanine, and leucine in the preparation Polyamine.
Добавил в систему: Шаповалова Елена Николаевна

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