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Receptor-mediated transport of foreign DNA into preimplantation mammalian embryosстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Ivanova M.M., Rosenkranz A.A., Smirnova O.A., Nikitin V.A., Sobolev A.S., Landa V., Naroditsky B.S., Ernst L.K.
- Журнал: Molecular Reproduction and Development
- Том: 54
- Номер: 2
- Год издания: 1999
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 112
- Последняя страница: 120
- DOI: 10.1002/(SICI)1098-2795(199910)54:2<112::AID-MRD2>3.0.CO;2-U
- Аннотация: Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine adenovirus). Video-intensified microscopy demonstrated that the constructs penetrated the zona pellucida and accumulated in the blastomere perinuclear space. The percentage of blastocysts formed was about 70% after incubation of zygotes and two-cell embryos with the constructs. Foreign DNA was detected after 51 hr in 80% of rabbit embryos and after 96 hr in 73% of mouse embryos. Inclusion of various adenoviruses into the construct improved foreign DNA preservation in early embryos. Blot hybridization revealed genome-integrated foreign DNA in 12- and iii-day mouse embryos and in a newborn. Thus, the ligand-mediated mechanism can be employed for introducing foreign genetic material into early mammalian embryos; insulin provides for delivery inside the cell and to the nucleus, while adenoviruses ensure release from endosomes. (C) 1999 Wiley-Liss, Inc.
- Добавил в систему: Розенкранц Андрей Александрович