Аннотация:For more than a century, Hylarana tytleri (Theobald, 1868) has been confused with two congeneric species, H. taipehensis and H. erythraea, in Bangladesh and neighboring countries, due to phenetic similarities as well as a lack of sufficient molecular and morphometric data. To resolve these problems, we conducted molecular and morphological surveys of Hylarana species throughout Bangladesh and examined H. taipehensis from Taiwan and Vietnam, "H. erythraea" from Vietnam, Thailand, Myanmar and Malaysia, and H. macrodactyla from China and Vietnam. Based on mitochondrial DNA sequence data, H. tytleri was shown to be genetically divergent from these relatives (7.4, 11.8 and 12.3 % for the 16S rRNA gene and 15.8, 19.8 and 20.9 % for HASAN et al. 2 the Cyt b gene in relation to H. macrodactyla, H. taipehensis and "H. erythraea", respectively). Morphologically, H. tytleri could also be distinguished from these congeners by the following: snout-vent length (SVL): 28.7-41.8 mm, rounded snout, moderately elongated head and off-white dorsolateral folds. Although H. tytleri showed the closest affinity with H. macrodactyla in the molecular phylogeny, the former is strikingly different in the absence of a middorsal stripe. In the future, these data may be useful as a reference to avoid erroneous species identification of Hylarana species in these regions.