Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Structural properties of nicked dsDNA have been an object of numerous studies due to their
special role in reparation processes. Here we report experimental results covering ultrasound
irradiation of a nicked dsDNA fragments. We have quantitatively estimated ultrasonic cleav-
age rates in these fragments using the polyacrylamide gel electrophoresis. Data reveal cleav-
age enhancement in the regions of about 10 b. p. up and down the nick. The intensity of
ultrasonic cleavage near the nick is one order of magnitude higher than intensity of ultra-
sonic cleavage in the same sites of the intact dsDNA fragments. At the same time, the cleav-
age rates in positions beyond the regions around the nick markedly grow weak comparing to
the sequence-specific cleavage rates of intact dsDNA. Thus, the presence of the nick serves
as an expressive structural alteration which exceeds any modulation of the structure caused
by the base-pair sequence.