Enzymatic method for the determination of inorganic and organic inhibitors of alcohol dehydrogenaseстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:The inhibiting effect of heavy metal ions, organic nitrogen-containing heterocyclic compounds, and amino acids on the catalytic activity of yeast alcohol dehydrogenase (ADH) in the reaction of ethanol oxidation by nicotinamide adenine dinucleotide was detected. Sensitive procedures were developed for the determination of the most effective ADH inhibitors [mercury(II), silver(I), zinc(II), copper(II), 1,10-phenanthroline, 2,2’-dipyridyl, 8-hydroxyquinoline, quinoline, 3,4-dimethylimidazole, benzimidazole, 1,2,3-benzotriazole, histidine, tryptophan, proline, and histamine] with c(L) = 5 x 10-10-1 x 10-4 M (RSD = 1-12%).