Место издания:Blackwell Publishing Inc United Kingdom
Первая страница:P13
Аннотация:Peptides are major signaling molecules in animals and yeasts. In plants, only a few peptides are known that control physiology, growth and development. Full sequencing of genome of the
moss Physcomitrella patens (Hedw.) B.S.G. together with recent
advances in peptide mass-spectrometry and bioinformatics opened
new possibilities for conducting genome-wide search of peptides
that perform signaling role in plants. In attempt to clarify the role
of peptides in moss physiology we started our studies on isolation,
identifi cation and further elucidation of physico-chemical and biological
properties of peptides from protoplasts, protonemata and
gametophores of Physcomitrella patens. We have elaborated the
strategy of extraction and fractionation of peptide-containing material
from moss protoplasts and tissues. Total mass-spectral analysis
by MALDI-TOF-TOF of HPLC fractions revealed over 400 individual
components with molecular masses below 10 kD. Individual
fractions were analyzed by LC-MS-MS FTI CR mass-spectrometry. A
number of moss endogenous peptides has been sequenced mainly
derived from chloroplast proteins.