Chemical reagents for immunodetection of fluoroquinolones with determinable specificity

Авторы: Shanin I.A., Zvereva E.A., Zherdev A.V., Godjevargova T., Eremin S.A.
Журнал: Bulgarian Chemical Communications
Том: 51
Номер: Special issue D
Год издания: 2019
Издательство: Bulgarian Chemical Society
Местоположение издательства: Bulgaria
Первая страница: 27
Последняя страница: 31
Аннотация: A new approach is proposed to obtain specific immunoreagents for detection of fluoroquinolones (FQs) that have a cyclopropyl radical in the 1st position of the quinolone core. The recommended immunogens should use coupling to a protein carrier via a carboxyl group in the 3rd position of the FQs, whereas solid-phase antigens for immunoassay should be obtained using the active groups of the radical in the 7th position. The given approach was applied to produce polyclonal antibodies against 3 FQs, danofloxacin (DAN), clinafloxacin (CLI), and moxifloxacin (MOX). The developed 15 enzyme immunoassays based on their use are characterized by detection limits of 0.5 ng/mL for DAN, 1.0 ng/mL for CLI, and 0.5 ng/mL for MOX. Interactions of the antibodies with 25 representatives of FQs were studied, and the possibility of using the obtained reagents for immunodetection of FQs with broad cross-reactivity was shown.
Добавил в систему: Еремин Сергей Александрович

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