Viral tRNA-like structures and pre-miRNA can mediate RNA phloem transportстатьяТезисы
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Дата последнего поиска статьи во внешних источниках: 23 января 2020 г.
Аннотация:The long-distance RNA transport in plants is shown to be essential for plant development and responses to different stresses; however molecular mechanisms of this process are largely
unknown. Recently, analyses of Cucurbita maxima phloem exudate revealed the presence of various RNA including mRNA, siRNA, miRNA, tRNA and rRNA. In addition, the functional significance of phloem transport for some RNAs such as miR399, miR395, miR827 and miR2111 was experimentally demonstrated. While, in general, signals in mobile RNAs enabling their phloem transport remain unknown, there are experimental data demonstrating significance of tRNA sequences embedded into the 30- terminal regions of some mRNAs. It is important to note that presence of tRNA sequences in many phloem-mobile mRNA correlates with the presence of tRNAs in the phloem sap. At the same time, tRNA-like structures (TLSs) are present at the 30-ends of genomic RNAs of certain plant
viruses with positive-stranded RNA genomes and potentially may enable their phloem transport. Here, we developed an experimental PVX-based system for visualization of RNA phloem transport
based on a modified potato virus X RNA capable of replication, but not encapsidation and movement in plants, and used this system for analysis of the ability of different RNAs to direct RNA phloem transport. We demonstrated that the Brome mosaic virus TLS, as well as the TLSs of Tobacco mosaic virus and Turnip yellow mosaic virus, contain signals of RNA systemic transport. Additionally, we revealed that unprocessed pre-miRNA can direct the phloem transport of heterologous RNA.