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[Isolation, purification, and characteristics of the new restriction enzymes BciBI and BciBII, produced by Bacillus circulans]статья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Samko O.T., Kalugin A.A., El’darov M.A., Karpychev I.V., Anike?cheva N.V., Khoroshutina E.B., Skriabin K.G., Librik G.I., Sokolov N.N.
- Журнал: Bioorganicheskaia khimiia
- Том: 20
- Номер: 12
- Год издания: 1994
- Первая страница: 1327
- Последняя страница: 1333
- Аннотация: New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification–by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10,000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively.
- Добавил в систему: Эльдаров Михаил Анатольевич