Kinetic determination of 2,4-dichlorophenoxyacetic acid by stopped-flow fluorescence polarization immunoassayстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:A kinetic metodology was applied to the determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by using fluorescence polarization immunoassay (FPIA). The analytical parameter used was the initial rate of the antigen-antibody reaction which was obtained from the kinetic curve degree of polarization-time and measured in only 1 s. The stopped-flow mixing technique was used for this purpose which allows the application of the method to routine analysis. The dynamic range of the calibration graph is 10-1000 ng ml(-1) and the detection limit is 4 ng ml(-1), lower than that afforded by the conventional FPIA. The relative standard deviation was close to 4%. The method was directly applied to the analysis of river water, grape juice and white wine and the analytical recoveries ranged from 92 to 110 %.