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Биотехнология экспрессной детекции биологического эффекта слабых электромагнитных воздействий / The Express Technology For The Biodetektion Of The Weak Electromagnetic Effectsстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 4 февраля 2014 г.
- Авторы: Мелехова О.П., Коссова Г.В., Падалка С.М., Сарапульцева Е.И., Padalka S.M.
- Сборник: Труды ХХ Международной конференции и дискуссионного научного клуба. «Новые информационные технологии в медицине, биологии, фармакологии и экологии»
- Год издания: 2012
- Место издания: Украина, Крым, Ялта-Гурзуф
- Первая страница: 235
- Последняя страница: 236
- DOI: УДК 577.346/591.39
- Аннотация: Abstract. The express technology for biological detection of the effects caused by small doses of electromagnetic irradiation (EMI) was developed, patented and put in practical use, at the Biology Department, Lomonosov MSU, in the expert assessing of ecological safety. The technology essence is that one assesses the adaptation stress of sensitive test objects early after their irradiation and then verifies the reliability of the assessment by watching postponed biological consequences. In this effort, we evaluated the oxidative stress semi-quantitatively—by watching a shift in the level of free-radical (FR) reactions revealed with the use of the radical copolymerization of 14C (AA-14C) radioactive indicator. Such an approach is approved by the high reactivity of metabolic free radicals, as well as by the oxidative stress of the bio-tests. Amphibian embryos of Xenopus laevis were our basic selected bio-test; they belonged to the laboratory line, i.e., were born and developed in standard conditions. Selecting the bio-tests of the proper age—the critical (most labile) stage of development—is a most important requirement. We invented this technology based on the fundamental research of the CR processes as involved in both epigenomic regulation of normal development and explicit response to damaging influences. When examining biological effects at their critical stage—neurula—we affected the amphibian embryos by -radiation of about 10-6 Gr dose from a 60Co source, by single X-ray irradiation to 0.3 dose, as well as by exposing to the irradiation, in complex, from a mobile telephone and a PC monitor for5 and 60 minutes, respectively. All these tests convincingly and reliably showed the level of CR reactions to change extensively and, next to it, reveal characteristically anomalous development and death of the embryos. Young daphnia (cladocera) subjected to small irradiation doses of 0.01 Gr, 0.1 Gr, and 1.0 Gr exhibited the reliably poor viability and altered level of CR reactions of the irradiated females as of their first breed. Thus, the EMI doses significantly smaller than those officially admitted limiting appeared critical, i.e., reducing vitality, for the embryo and young hydrobionts. Ecological consequences of the chronic weak EMI influences in vivo is that they diminish the hydrobiont populations and disturb ecological balance.
- Добавил в систему: Мелехова Ольга Петровна