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Effect of Na+ and K+ ions on the luminescence of intact Vibrio harveyi cells at different pH valuesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Vitukhnovskaya L.A., Ismailov A.D.
- Журнал: Microbiology
- Том: 70
- Номер: 4
- Год издания: 2001
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 456
- Последняя страница: 461
- DOI: 10.1023/A:1010490312650
- Аннотация: The bioluminescent activity of intact Vibrio harveyi cells loaded with different concentrations of NaCl and KCl at different pH values was studied. In the pH range of 6.5-8.5, the effect of Na+ was significantly higher than that of K+ at all concentrations studied. Maximum luminescent activity was observed in cells loaded with 0.68 M NaCl. When Na+ was nonuniformly distributed on the plasma membrane, the cell luminescence kinetics was nonstationary in the 20-min range: during incubation, the luminescence intensity increased at pH 6.5 and decreased at pH 8.5. The activation and damping rate constants depended on the Na+ gradient value. The maximum of luminescent activity shifted during incubation from pH 8.5 to 6.5-7.0. The luminescence kinetics in the systems with KCI was stationary; the maximum level of luminescence was observed in the pH range of 7.0-7.5. Under Na+-controlled conditions, the cell respiration and luminescence changed in synchronism. The protonophore CCP at a concentration of 20 muM completely inhibited luminescence at pH 6.5 and was ineffective at pH 8.5.
- Добавил в систему: Исмаилов Анвар Джураевич