Factors influencing the intensity of luminescence of Photobacterium phosphoreum grown in submerged cultureстатья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Местоположение издательства:Road Town, United Kingdom
Первая страница:148
Последняя страница:152
Аннотация:Aliphatic aldehydes decanal, tetradecanal, and cis-11-hexadecenal were analyzed for their ability to affect the luminescence of Photobacterium phosphoreum cells taken at the stages of maximum luminescence and luminescence decay. The decay of luminescence was found to be related neither to the aldehyde limitation of luciferase nor to the accumulation of the products of lipid peroxidation. Comparative analysis of NAD(P)H:FMN oxidoreductase activity in cell-free extracts showed that this activity was 3-4 times higher in cells at the stage of maximum luminescence than in the cells at the stage of luminescence decay. It is suggested that one of the factors responsible for the decrease in the luminescence of photobacteria grown in submerged culture is the uncoupling of the light-producing electron transport chain at the stage of reduction of a flavin substrate of luciferase.