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Recombinant modular transporters on the basis of epidermal growth factor for targeted intracellular delivery of photosensitizersстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Gilyazova D.G., Rosenkranz A.A., Gulak P.V., Lunin V.G., Sergienko O.V., Grin M.A., Mironov A.F., Rubin A.B., Sobolev A.S.
- Сборник: Proceedings of SPIE - The International Society for Optical Engineering
- Год издания: 2005
- Первая страница: 101
- Последняя страница: 110
- DOI: 10.11
- Аннотация: The search for new pharmaceuticals has raised interest in local ly-acting drugs which act over short distances within the cell , and for which different c ell compartments b ave different sensitivities. T hus, p botosensitizers u sed i n a nti-cancer therapy should be tra nspo rted to the m ost s ensitive s ubcellular compamnents where t heir a ction i s most pronounced. Earl ier, we described the effects of bacterially expressed modular recombinant transport ers for pbotosensitizers comprising a-melanocyte• stimulating hormone as an intemalizable, cell-spec ific ligand, an optimized nuclear localization sequence, an Escherichia coli hemoglobin-like protein as a carrier, and an endosomolytic arnp hipa thic polypeptide. These transporters delivered photose nsitizers into the murine melanoma cells nuclei to resul t in cyto toxic effe cts 2 orders of magnitude greater than those of nonmodified photosensidzers. Here we d escribe n ew transport ers possessing the same modules excep t for a ligand that is repl aced with epiderma l growth factor specific for other cancer cell types . The new tra nsporter modules retained their functional activities within the chi mera, this transporter delivered photosensitizers into the human carcinoma cells nuclei to result in pbotocyt croxic effects almost 3 orders of magnitude greater than those of nonmodified photoseasirizers. The obtained results show that ligand modules of such transporters are interchangeable, meaning that they can be tailored for parti cular appli cations.
- Добавил в систему: Розенкранц Андрей Александрович